The Skaro meanies first appeared in the show’s second story in 1963, doing battle with William Hartnell’s Doctor and his granddaughter Susan.
They’re returned to appear opposite almost all Doctors, becoming a staple of the series and its most famous alien race.
The anniversary special stars Matt Smith, David Tennant and John Hurt as the Doctor and will be broadcast on November 23rd, the same day the series debuted in 1963.
Hurt was unveiled as a mysterious past Doctor at the end of the latest season.
The Doctors will be joined by former companion Billie Piper, Jenna Coleman as current companion Clara Oswald and Gavin and Stacey star Joanne Page.
Also confirmed to appear are the Zygons, a shape shifting alien race who previously appeared with Tom Baker’s Doctor in 1975.
Confirming the return of the Daleks, Steven Moffat, lead writer and executive producer, said: “The Doctor once said that you can judge a man by the quality of his enemies, so it’s fitting that for this very special episode, he should facing the greatest enemies of all.”