The original series ran for 26 episodes across four series between 1973 and 1985.
The show starred Ronnie Barker as shopkeeper Arkwright, Sir David Jason as nephew Granville and Lynda Baron as nurse Gladys, the target of Arkwright’s affections.
Jason and Baron will reprise their roles for the special alongside Maggie Ollerenshaw who played Mavis for seven episodes of the original run.
In the new episode, written by original writer Roy Clarke, Granville has inherited the back-water grocery store from Arkwright and now runs it.
David Jason said: “I’m really excited to be bringing back Open All Hours. I am sure there is an audience out there who would like to see what Granville has been getting up to in the corner shop.
“It will be a great family show for Christmas and a fitting tribute to the legacy of Arkwright.”
BBC Controller of UK Comedy Production, Mark Freeland, says: “I am absolutely delighted to welcome back to BBC comedy the magic pairing of David Jason and Roy Clarke. Having the chance to see Arkwright’s beloved shop again, now run by Granville, I hope will bring broad grins to lots and lots of faces at Christmas.”