The 75-minute special saw outgoing Doctor Matt Smith partner with predecessor David Tennant and a mysterious previous incarnation played by John Hurt.
Spoilers:
The three Doctors joined forces in an effort to save their home planet Gallifrey from being destroyed in the Time War, the epic battle between the Time Lords and Daleks which was introduced by Russell T Davies when he revived the series in 2005.
Previous Doctors including William Hartnell, Peter Davison and Colin Baker also appeared through the use of archive footage while Tom Baker, the show’s longest serving star and the earliest surviving Doctor, appeared opposite Smith as a much older version of the Fourth Doctor.
The episode was shown simultaneously in 94 countries across 6 continents and in over 1500 cinemas worldwide with more than half a million tickets sold.
In the UK the special was seen by more than 10m viewers on BBC One and BBC One HD.
Craig Glenday, Editor-in-Chief of Guinness World Records, presented the award to the show’s Head Writer Steven Moffat on the final day of the BBC’s Doctor Who Celebration event at London’s ExCel.
Glenday said: “Who else but the time-twisting Doctor could appear in 94 countries at once?! This outstanding achievement is testament to the fact that the longest running sci-fi TV show in history is not just a well-loved UK institution but a truly global success adored by millions of people.”
Accepting the award, Moffat said: “For years the Doctor has been stopping everyone else from conquering the world. Now, just to show off, he’s gone and done it himself!”
Doctor Who: The Day of the Doctor is released on Blu-ray and DVD on December 2nd and is available to pre-order from Amazon.co.uk.